Abstract
Collagen hydrolysates in dietary supplements are sources of amino acids and di- and tripeptides. They are often used to support connective tissue metabolism; however, data on their bioavailability are limited.
Objective. To investigate the absorption efficacy of oral collagen hydrolysate (First Alive Collagen) by analyzing dynamic shifts in the amino acid composition of blood plasma and urine as markers of connective tissue metabolism.
Material and methods. This prospective, randomized, placebo-controlled study included 30 healthy volunteers allocated into two groups. The mean age was 33±7.8 years for men and 32±8.1 years for women. The main group (n=15) received 20 g per day of collagen hydrolysate (First Alive Collagen). The control group (n=15) received a placebo. Fasting blood plasma and urine samples were collected at baseline (day 0) and after three weeks (day 21).
Results. The main group showed a significant increase in plasma levels of hydroxyproline (+37%, p<0.001), proline (+24%, p=0.002), alanine (+17%, p=0.003), and glycine (+9%, p=0.021). Urinary excretion decreased for hydroxyproline (-29%, p<0.001), proline (-19%, p=0.004), glycine (-12%, p=0.015), and alanine (-23%, p=0.001). A significant increase in the levels of other plasma amino acids (glutamine, arginine, serine, threonine) was also observed. No significant changes were observed in the control group (p>0.05 for all amino acid profile indicators). Significant «group × time» interaction effects were found for plasma (F=28.4, p<0.001, η2=0.51) and urinary (F=22.7, p<0.001, η2=0.45) hydroxyproline.
Conclusion. Supplementation with collagen hydrolysate (First Alive Collagen) significantly increases the level of specific collagen amino acids in plasma and reduces their urinary excretion, confirming its bioavailability and metabolic utilization. The synchronous increase in plasma concentration and decrease in urinary excretion of collagen-specific amino acids indicates effective absorption, reduced catabolism, and active incorporation of hydrolysate peptides into metabolism.
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Alexey D. Repetyuk, Konstantin S. Ternovoi, Sergey A. Artemyev, Irina A. Artemyeva, Shelekhova Tatiyana Yu, Anna M. Belyakova, Svetlana Yu. Shokur, The effect of oral collagen hydrolysate intake on the dynamics of blood plasma and urine amino acid composition as markers of its bioavailability and the synthesis of endogenous collagen, https://endocrinology-journal.ru/en/jarticles_endo/978.html?SSr=07E90C172FCA1
